产品说明
Cell line:DU-145 DSMZ no.:ACC 261 Species:human (Homo sapiens) Cell type:prostate carcinoma Origin: established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 Biosafety level:1 Permissions and restrictions:A DSMZ Cell Culture Data: Morphology:epithelial-like adherent cells growing as monolayers Medium:90% RPMI 1640 + 10%FBS Subculture:split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 Incubation:at 37 °C with 5% CO2 Doubling time:about 30-40 hours Harvest:cell harvest of about 35 x 106 cells/175 cm2 Storage:frozen with 70% medium, 20% FBS, 10% DMSO
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