产品说明
Cell line:LNCAP DSMZ no.:ACC 256 Species:human (Homo sapiens) Cell type:prostate carcinoma Origin: established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described to be androgen-sensitive Biosafety level:1 Permissions and restrictions:A DSMZ Cell Culture Data: Morphology:adherent fibroblastoid cells growing in aggregates and as single cells Medium:80% RPMI 1640 + 20% FBS Subculture: split semi-confluent culture 1:4 once a week using trypsin/EDTA (occasionally cells can be detached by tapping); seed out at ca. 1-2 x 106 cells/25 cm2; after thawing and trypsinization cells may need 1-2 days to become adherent again; due to the strong cell aggregation, it might be difficult to determine an exact cell titer Incubation: at 37 °C with 5% CO2 Doubling time:about 60 hours Harvest:cell harvest of about 10-15 x 106 cells/80 cm2 flask Storage: frozen with 70% medium, 20% FBS, 10% DMSO
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