产品说明
Cell line:PC-3 DSMZ no:ACC 465 Species:human (Homo sapiens) Cell type:prostate carcinoma Origin: established from the bone marrow metastasis isolated post-mortem from a 62-year-old Caucasian man with grade IV prostate cancer (poorly differentiated adenocarcinoma) after androgen suppression therapy; described to form tumors in nude mice, to grow in soft agar, and to be unresponsive to androgen treatment Biosafety level:1 Permissions and restrictions:A DSMZ Cell Culture Data:
Morphology: epithelial-like cells growing adherently in monolayers or in multilayer foci Medium: 45% Ham's F12 + 45% RPMI 1640 + 10% FBS F-12K Medium（Catalog No. 30-2004）+10%fetal bovine serum
Subculture:split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; seed out at ca. 1 x 104 cells/cm2 Incubation:at 37 °C with 5% CO2 Doubling time:ca. 50 hours Harvest:cell harvest of ca. 0.5 x 105 cells/cm2 Storage:frozen with 70% medium, 20% FBS, 10% DMSO
