B-CPAP/Bcpap，人甲状腺癌细胞 细胞图片

Origin:
established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992
Biosafety level:1
Permissions and restrictions:A
Morphology: spindle- or round-shaped cells growing adherently as monolayer
Medium:90% RPMI 1640 + 10%FBS
Subculture:
 split confluent culture 1:4 to 1:6 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 10⁶ cells/80 cm²
Incubation:at 37 °C with 5% CO₂
Doubling time:about 30 hours
Harvest:about 10 x 10⁶ cells/80 cm²
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO