Knock-out any gene or edit the genome with this HR targeting vector—features an RFP marker and puromycin selection as a removable selection cassette.

Overview

Get precise genomic integration of your expression cassette

Use the PrecisionX^TM Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Puro-pA-MCS2) to knock-out any gene or edit the genome. Clone your homology arms into MCS1 and MCS2, and use puromycin selection and RFP-positive imaging to find integrants. After you’ve identified clones with your gene-of-interest knocked-out or edited, you can remove the selection cassette using the Cre-LoxP system (learn more about Cre-LoxP excision here).

Why use an HR targeting vector?

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.

Choose the right HR Targeting Vector for your project