ExoFACS™ Kit for Cell Culture Media Exosomes K1236-20-生化试剂-上海觅拓生物科技有限公司

生化试剂

ExoFACS™ Kit for Cell Culture Media Exosomes K1236-20

产品介绍【返回】

货号：	K1236-20
保存条件：	4度
供应商：	上海觅拓生物
英文名：	ExoFACS™ Kit for Cell Culture Media Exosomes
数量：	3
规格：	20次

Introduction:

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

ExoFACS™ allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. The kit consists of components for exosome isolation, 4 µm beads for the overall capture of pre-isolated exosomes and lyophilized exosomes from cell culture supernatants or human biological fluids as the positive control. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens. Biovision offers different ExoFACS™ kits for staining of exosomal markers from human biofluids (plasma, urine, serum, saliva) and from cell culture supernatants. ExoFACS™ contains reagents for 20 reactions (lyophilized exosomes, beads, antibodies and buffers). Primary antibody included in the kit is against a common exosomal marker (CD9 or CD63) and can be used as a positive control for protein profiling via FACS analysis.

Figure 1. ExoFACSTM Exosome isolation and exosome marker characterization via FACS

Application:
- ExoFACS™ Kits ensure exosome isolation and exosome marker characterization via FACS
- Exosome comprehensive profiling
- No initial exosome purification required
- Lyophilized Exosome Standards for positive control included
- User friendly and suitable for multiple marker analyses
Sample Type:
- Human biological fluids: Cell culture media.
Kit Contents (Ready to use kit for FACS analysis from cell media):

Components	Description	K1236-20	Part Number
ExoPureTM Reagent FACS-beads Primary Antibody Secondary Antibody Sample Buffer (5X) Exosome Standards (100 µg)	Exosome Isolation Reagent 4 µm Aldehyde-Sulfate latex beads Anti-human CD63 mouse antibody Secondary antibody Alexa 488 Buffer for antibody incubation Lyophilized exosomes (from Cell Lines)	1 bottle (20 ml) 1 vial (100 μl) 1 vial (20 μl) 1 vial (3 μl) 1 bottle (10 ml)) 1 vial (100 μg)	K1236-20-1 K1236-20-2 K1236-20-3 K1236-20-4 K1236-20-5 K1236-20-6

User Supplied Reagents and Equipment:
- Single-use and/or pipettes with disposable tips 2-100 μl
- Polypropylene tubes
- Pipettes 1 ml and 5 ml for reagent preparation
- Deionized water
- PBS
- BSA or FBS or FCS
- Disposable pipetting reservoirs
- Prepare the Washing buffer (not provided in the kit)
- FACS tubes
Shipment and Storage:

All the reagents are shipped and stored at 4°C for up to 12 months, if unopened. Briefly centrifuge small vials prior to opening. DO NOT FREEZE!

Reagent Preparation and Storage Conditions:

ExoFACSTM contains reagents and antibodies for 20 reactions.
Exosome standards must be reconstituted in 100 µl of deionized water.
Isolation Component is included in the kit for exosome isolation
Beads are ready to use for exosome capture.
Primary and secondary antibody must be appropriately diluted in sample buffer.
1 vial (100 µg) of exosome standards (lyophilized), (possibility to choose from the list of lyophilized exosome standards from cell media available (number of particles/ml 1x1010)).
Exosome standards: The remaining reconstituted standard stock solution should be aliquoted into polypropylene vials (preferably low binding) and stored at -20°C for up to one month or at -80°C for up to six months. Strictly avoid repeated freeze-and-thaw cycles.
Store opened and diluted reagents at 4°C up to 12 months if unopened.

上一篇：

Sigma，GW4869，D1692 外泌体抑制剂

下一篇：

QIAGEN ,77023/ 77044/77064,exoRNeasy Serum/Plasma Maxi Kit Print,一小时提取外泌体中RNA

服务热线：18616635231

生化试剂

ExoFACS™ Kit for Cell Culture Media Exosomes K1236-20

产品介绍【返回】

友情链接