Beta-TC-6 小鼠胰岛素瘤胰岛β细胞 ATCC® CRL-11506™-细胞产品-上海觅拓生物科技有限公司

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产品货号	英文简称	产品名称	规格	价格
CRL-11506™	Beta-TC-6	Beta-TC-6 小鼠胰岛素瘤胰岛β细胞 ATCC® CRL-11506™	T25	1350

产品说明

货号：	CRL-11506™
供应商：	上海觅拓生物
规格：	T25

Organism	Mus musculus, transgenic for SV40 large T antigen, mouse, transgenic for SV40 large T antigen
Tissue	pancreas
Cell Type	beta cell
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 [Cells contain SV40 viral DNA Sequences] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	insulinoma
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation	The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse.
Genes Expressed	insulin, glucagon, and somatostatin
Cellular Products	insulin, glucagon and somatostatin
Comments	They secrete insulin in response to glucose. The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promotor. The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C

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