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AP染色试剂盒 Alkaline Phosphatase Detection Kit

产品介绍【返回】

货号: SCR004
供应商: 上海觅拓
英文名: Alkaline Phosphatase Detection Kit
规格: 100T


性质

Related Categories 抗体抗体试剂盒 更多...
trade name   Chemicon
brand family   Chemicon
application(s)   Stem Cell Culture
stem cell type   Mouse Embryonic Stem Cells
  Human Embryonic Stem Cells
  Mesenchymal Stem Cells
  Neural Stem Cells
  Hematopoietic Stem Cells
  Epithelial Cells
  Pancreatic Stem Cells
  Cardiac Stem Cells
  Induced Pluripotent Stem Cells
species reactivity   Mammals
material size   100 assays
shipped in   wet ice
storage conditions   The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity. 

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

 

产品描述

Other Notes

Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Components

Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.

Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.

materials required but not delivered

1. Fixative (e.g. 4% Paraformaldehyde in PBS) 

2. 1 x Rinse Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15M NaCl, 0.05% Tween-20) 

3. Hematoxylin (optional) 

4. Microscope

Application

Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4. 

Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity. 

Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7]. 

For Research Use Only; Not for use in diagnostic procedures

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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