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MEM α, no Nucleosides 12561056

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货号: 12561056
英文名: MEM α, no Nucleosides
规格: 500ml

描述

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM α is modified as follows:

With Without
• Phenol Red • Ribonucleosides
• L-glutamine • Deoxyribonucleosides


The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM α product made in our Scotland facility (22561-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Glucose: Low Glucose
Product Line: Gibco®
Product Size: 500 mL
Serum Supplementation: Standard Serum Supplementation
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature
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