Cell line:NB-4
DSMZ no.:ACC 207
Species:human (Homo sapiens)
Cell type:acute promyelocytic leukemia
Origin:
established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene
Biosafety level:1
Permissions and restrictions:A
Morphology:
single cells in suspension, round and polymorphic cells, very few are giant cells;Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 10⁶ cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5-1 x 10⁶ cells/ml; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks
Incubation:at 37 °C with 5% CO₂
Doubling time:ca. 35-45 hours
Harvest:cell harvest of 1.5-2.0 x 10⁶cells/ml
Storage:frozen with 70% medium, 20% FBS, 10% DMSO