Origin: 
    established from the peripheral blood of a 7-year-old Japanese boy with acute myeloid leukemia (AML FAB M2) (in 2nd relapse after bone marrow transplantation) in 1989; cells carry the t(8;21) leading to RUNX1-RUNX1T1 (AML1-ETO) fusion gene; described to carry the KIT mutation N822
Biosafety level: 1
Risk assessment: 
    The cell line was shown to exhibit reverse transcriptase (RT) activity detected by RT-ELISA. However, HIV-1 and -2 or HTLV-I and -II sequences could not be detected by PCR analysis. The cell line is thus categorized biosafety level 1.
Permissions and restrictions: A
 
Medium: 80% RPMI 1640 + 20% FBS
Subculture: 
     in log-phase, optimal density is at about 0.5 x 106 cells/ml and a split ratio of saturated culture about 1:2 every 2-3 days; viability may drop below 50% within the first 24 hours after thawing; we recommend to start culture with a cell concentration of >1 x 106 cells/ml and to cultivate initially in 24-well plates; a significant amount of cell debris is always visible in the background which might be due to granula or other intracellular material
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 48-72 hours
Harvest: saturation density at about 1.0 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO 
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